FineTest
SKU:EH3694
Human Rac-GAP1(Rac-GTPase Activating Protein 1) ELISA Kit
Human Rac-GAP1(Rac-GTPase Activating Protein 1) ELISA Kit
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Product Name
Human Rac-GAP1(Rac-GTPase Activating Protein 1) ELISA Kit
Alias
Rac-GAP1 ELISA Kit, Rac-GTPase Activating Protein 1 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Rac-GAP1 ELISA Kit allows for the in vitro quantitative determination of Rac-GAP1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
78.125-5000pg/ml
Sensitivity
< 46.875pg/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Rac-GAP1 and the recovery rates were calculated by comparing the measured value to the expected amount of Rac-GAP1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
86-105 |
97 |
|
EDTA plasma(n=5) |
86-105 |
93 |
|
heparin plasma(n=5) |
87-105 |
93 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Rac-GAP1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
88-103% |
89-105% |
86-105% |
|
EDTA plasma(n=5) |
86-101% |
82-97% |
83-98% |
|
heparin plasma(n=5) |
83-98% |
87-97% |
82-88% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
