FineTest
SKU:EH3625
Human pNF-H(Phosphorylated Neurofilament H) ELISA Kit
Human pNF-H(Phosphorylated Neurofilament H) ELISA Kit
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Product Name
Human pNF-H(Phosphorylated Neurofilament H) ELISA Kit
Alias
pNF-H ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
pNF-H ELISA Kit allows for the in vitro quantitative determination of pNF-H? concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
78.125-5000pg/ml
Sensitivity
< 46.875pg/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of pNF-H? and the recovery rates were calculated by comparing the measured value to the expected amount of pNF-H? in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
91-103 |
95 |
|
EDTA plasma(n=5) |
87-103 |
97 |
|
heparin plasma(n=5) |
85-100 |
92 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of pNF-H? and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-100% |
94-101% |
91-103% |
|
EDTA plasma(n=5) |
83-101% |
88-101% |
83-99% |
|
heparin plasma(n=5) |
84-100% |
80-100% |
82-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
