FineTest
SKU:EH3604
Human PLA2G12B(Phospholipase A2, Group XII B) ELISA Kit
Human PLA2G12B(Phospholipase A2, Group XII B) ELISA Kit
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Product Name
Human PLA2G12B(Phospholipase A2, Group XII B) ELISA Kit
Alias
PLA2G12B ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
PLA2G12B ELISA Kit allows for the in vitro quantitative determination of PLA2G12B concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of PLA2G12B and the recovery rates were calculated by comparing the measured value to the expected amount of PLA2G12B in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
86-104 |
95 |
|
EDTA plasma(n=5) |
89-103 |
97 |
|
heparin plasma(n=5) |
88-99 |
93 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PLA2G12B and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
91-104% |
85-90% |
85-102% |
|
EDTA plasma(n=5) |
89-101% |
82-96% |
83-95% |
|
heparin plasma(n=5) |
88-100% |
90-97% |
83-97% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
