FineTest
SKU:EH3591
Human PKCη(Protein Kinase C Eta) ELISA Kit
Human PKCη(Protein Kinase C Eta) ELISA Kit
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Product Name
Human PKCη(Protein Kinase C Eta) ELISA Kit
Alias
PKCη ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
PKCη ELISA Kit allows for the in vitro quantitative determination of PKCη concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of PKCη and the recovery rates were calculated by comparing the measured value to the expected amount of PKCη in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-101 |
94 |
|
EDTA plasma(n=5) |
87-101 |
96 |
|
heparin plasma(n=5) |
86-103 |
94 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PKCη and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-104% |
90-102% |
89-105% |
|
EDTA plasma(n=5) |
88-94% |
82-94% |
87-99% |
|
heparin plasma(n=5) |
80-97% |
83-98% |
83-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
