FineTest
SKU:EH3537
Human PDHα(Pyruvate Dehydrogenase Alpha) ELISA Kit
Human PDHα(Pyruvate Dehydrogenase Alpha) ELISA Kit
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Product Name
Human PDHα(Pyruvate Dehydrogenase Alpha) ELISA Kit
Alias
PDHα ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
PDHα ELISA Kit allows for the in vitro quantitative determination of PDHα concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of PDHα and the recovery rates were calculated by comparing the measured value to the expected amount of PDHα in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-105 |
96 |
|
EDTA plasma(n=5) |
88-104 |
98 |
|
heparin plasma(n=5) |
86-103 |
98 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PDHα and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
88-102% |
91-105% |
87-98% |
|
EDTA plasma(n=5) |
82-101% |
86-97% |
85-98% |
|
heparin plasma(n=5) |
81-96% |
82-84% |
84-95% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
