FineTest
SKU:EH3532
Human PDGF-AA(Platelet Derived Growth Factor-AA) ELISA Kit
Human PDGF-AA(Platelet Derived Growth Factor-AA) ELISA Kit
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Product Name
Human PDGF-AA(Platelet Derived Growth Factor-AA) ELISA Kit
Alias
PDGF-AA ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
PDGF-AA ELISA Kit allows for the in vitro quantitative determination of PDGF-AA concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Human
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of PDGF-AA and the recovery rates were calculated by comparing the measured value to the expected amount of PDGF-AA in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-101 |
92 |
|
EDTA plasma(n=5) |
88-104 |
96 |
|
heparin plasma(n=5) |
89-104 |
98 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PDGF-AA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
91-103% |
88-98% |
91-102% |
|
EDTA plasma(n=5) |
87-99% |
86-97% |
89-101% |
|
heparin plasma(n=5) |
81-95% |
81-95% |
84-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
