FineTest
SKU:EH3529
Human PDCN(Podocin) ELISA Kit
Human PDCN(Podocin) ELISA Kit
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Product Name
Human PDCN(Podocin) ELISA Kit
Alias
PDCN ELISA Kit, NPHS2 ELISA Kit, Podocin ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
PDCN ELISA Kit allows for the in vitro quantitative determination of PDCN concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
78.125-5000pg/ml
Sensitivity
< 46.875pg/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of PDCN and the recovery rates were calculated by comparing the measured value to the expected amount of PDCN in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
87-105 |
94 |
|
EDTA plasma(n=5) |
89-103 |
96 |
|
heparin plasma(n=5) |
93-104 |
99 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PDCN and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-104% |
88-104% |
85-100% |
|
EDTA plasma(n=5) |
83-101% |
86-95% |
89-97% |
|
heparin plasma(n=5) |
88-100% |
86-96% |
85-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
