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SKU:EH3478

Human ON(Osteonectin) ELISA Kit

Human ON(Osteonectin) ELISA Kit

Regular price $450.00 USD
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size
Sensitivity
Range

Product Name

Human ON(Osteonectin) ELISA Kit

Alias

ON ELISA Kit, BM-40 ELISA Kit, Basement-membrane protein 40 ELISA Kit, BM-40 ELISA Kit, ONcysteine-rich protein ELISA Kit, Secreted protein acidic and rich in cysteine ELISA Kit, secreted protein ELISA Kit, acidic ELISA Kit, cysteine-rich ELISA Kit, osteonectin ELISA Kit

Detection Method

Sandwich ELISA, Double Antibody

Application

ON ELISA Kit allows for the in vitro quantitative determination of ON concentrations in serum, plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Species

Human

UniProt ID

P09486

Standard Curve

Storage

2-8 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of ON and the recovery rates were calculated by comparing the measured value to the expected amount of ON in samples.

Matrix

Recovery range(%)

Average(%)

serum(n=5)

87-104

98

EDTA plasma(n=5)

86-104

97

heparin plasma(n=5)

86-102

94

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ON and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

serum(n=5)

85-102%

86-105%

88-103%

EDTA plasma(n=5)

89-91%

82-91%

82-99%

heparin plasma(n=5)

83-100%

85-98%

83-95%

CV(%)

Intra-Assay: CV<8%

Inter-Assay: CV<10%

Note

For Research Use Only

 

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