FineTest
SKU:EH3478
Human ON(Osteonectin) ELISA Kit
Human ON(Osteonectin) ELISA Kit
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Product Name
Human ON(Osteonectin) ELISA Kit
Alias
ON ELISA Kit, BM-40 ELISA Kit, Basement-membrane protein 40 ELISA Kit, BM-40 ELISA Kit, ONcysteine-rich protein ELISA Kit, Secreted protein acidic and rich in cysteine ELISA Kit, secreted protein ELISA Kit, acidic ELISA Kit, cysteine-rich ELISA Kit, osteonectin ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
ON ELISA Kit allows for the in vitro quantitative determination of ON concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Human
UniProt ID
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of ON and the recovery rates were calculated by comparing the measured value to the expected amount of ON in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
87-104 |
98 |
|
EDTA plasma(n=5) |
86-104 |
97 |
|
heparin plasma(n=5) |
86-102 |
94 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ON and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-102% |
86-105% |
88-103% |
|
EDTA plasma(n=5) |
89-91% |
82-91% |
82-99% |
|
heparin plasma(n=5) |
83-100% |
85-98% |
83-95% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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