FineTest
SKU:EH3429
Human N-MID-OT(N-MID Osteocalcin) ELISA Kit
Human N-MID-OT(N-MID Osteocalcin) ELISA Kit
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Product Name
Human N-MID-OT(N-MID Osteocalcin) ELISA Kit
Alias
N-MID-OT ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
N-MID-OT ELISA Kit allows for the in vitro quantitative determination of N-MID-OT concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.625-40ng/ml
Sensitivity
< 0.375ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of N-MID-OT and the recovery rates were calculated by comparing the measured value to the expected amount of N-MID-OT in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
92-104 |
99 |
|
EDTA plasma(n=5) |
88-99 |
95 |
|
heparin plasma(n=5) |
89-105 |
95 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of N-MID-OT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
88-102% |
86-98% |
88-105% |
|
EDTA plasma(n=5) |
87-99% |
88-98% |
84-99% |
|
heparin plasma(n=5) |
89-99% |
90-99% |
81-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
