FineTest
SKU:EH3325
Human MAPK(Mitogen Activated Protein Kinase) ELISA Kit
Human MAPK(Mitogen Activated Protein Kinase) ELISA Kit
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Product Name
Human MAPK(Mitogen Activated Protein Kinase) ELISA Kit
Alias
MAPK ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
MAPK ELISA Kit allows for the in vitro quantitative determination of MAPK concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
1.563-100ng/ml
Sensitivity
< 0.938ng/ml
Species
Human
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of MAPK and the recovery rates were calculated by comparing the measured value to the expected amount of MAPK in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
93-105 |
98 |
|
EDTA plasma(n=5) |
93-104 |
96 |
|
heparin plasma(n=5) |
86-99 |
93 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MAPK and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
87-104% |
86-103% |
87-101% |
|
EDTA plasma(n=5) |
84-100% |
87-100% |
91-100% |
|
heparin plasma(n=5) |
81-96% |
80-97% |
88-94% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
