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SKU:EH3322
Human MANF(Mesencephalic Astrocyte Derived Neurotrophic Factor) ELISA Kit
Human MANF(Mesencephalic Astrocyte Derived Neurotrophic Factor) ELISA Kit
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Product Name
Human MANF(Mesencephalic Astrocyte Derived Neurotrophic Factor) ELISA Kit
Alias
MANF ELISA Kit, ARMET ELISA Kit, ARP ELISA Kit, Arginine-Rich Protein ELISA Kit, arginine-rich ELISA Kit, mutated in early stage tumors ELISA Kit, ARMET ELISA Kit, ARP ELISA Kit, mesencephalic astrocyte-derived neurotrophic factor ELISA Kit, Protein ARMET ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
MANF ELISA Kit allows for the in vitro quantitative determination of MANF concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Human
UniProt ID
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of MANF and the recovery rates were calculated by comparing the measured value to the expected amount of MANF in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
89-103 |
98 |
|
EDTA plasma(n=5) |
86-105 |
95 |
|
heparin plasma(n=5) |
94-105 |
100 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MANF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-102% |
87-101% |
90-105% |
|
EDTA plasma(n=5) |
84-95% |
85-97% |
84-97% |
|
heparin plasma(n=5) |
85-99% |
84-98% |
82-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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