FineTest
SKU:EH3263
Human IgG4(Immunoglobulin G4) ELISA Kit
Human IgG4(Immunoglobulin G4) ELISA Kit
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Product Name
Human IgG4(Immunoglobulin G4) ELISA Kit
Alias
IgG4 ELISA Kit, Immunoglobulin G4 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
IgG4 ELISA Kit allows for the in vitro quantitative determination of IgG4 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
6.25-400pg/ml
Sensitivity
< 3.75pg/ml
Species
Human
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of IgG4 and the recovery rates were calculated by comparing the measured value to the expected amount of IgG4 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
86-104 |
95 |
|
EDTA plasma(n=5) |
86-105 |
96 |
|
heparin plasma(n=5) |
85-103 |
94 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IgG4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-97% |
85-101% |
97-105% |
|
EDTA plasma(n=5) |
90-99% |
84-98% |
85-94% |
|
heparin plasma(n=5) |
80-99% |
92-98% |
80-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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