FineTest
SKU:EH3252
Human IFN-α(Interferon Alpha) ELISA Kit
Human IFN-α(Interferon Alpha) ELISA Kit
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Product Name
Human IFN-α(Interferon Alpha) ELISA Kit
Alias
IFN-α ELISA Kit, Interferon Alpha ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
IFN-α ELISA Kit allows for the in vitro quantitative determination of IFN-α concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Human
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of IFN-α and the recovery rates were calculated by comparing the measured value to the expected amount of IFN-α in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
89-105 |
95 |
|
EDTA plasma(n=5) |
85-104 |
95 |
|
heparin plasma(n=5) |
87-100 |
94 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IFN-α and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-94% |
86-104% |
92-105% |
|
EDTA plasma(n=5) |
83-98% |
85-100% |
86-101% |
|
heparin plasma(n=5) |
80-95% |
81-96% |
80-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
