FineTest
SKU:EH3250
Human IER3(Immediate Early Response 3) ELISA Kit
Human IER3(Immediate Early Response 3) ELISA Kit
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Product Name
Human IER3(Immediate Early Response 3) ELISA Kit
Alias
IER3 ELISA Kit, PACAP-responsive gene 1 protein ELISA Kit, Protein PRG1 ELISA Kit, Immediate early response 3 protein ELISA Kit, Differentiation-dependent gene 2 protein ELISA Kit, Protein DIF-2 ELISA Kit, Immediate early protein GLY96 ELISA Kit, Radiation-inducible immediate-early gene IEX-1 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
IER3 ELISA Kit allows for the in vitro quantitative determination of IER3 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
78.125-5000pg/ml
Sensitivity
< 46.875pg/ml
Species
Human
UniProt ID
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of IER3 and the recovery rates were calculated by comparing the measured value to the expected amount of IER3 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-105 |
95 |
|
EDTA plasma(n=5) |
89-103 |
95 |
|
heparin plasma(n=5) |
85-102 |
97 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IER3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-99% |
87-102% |
89-100% |
|
EDTA plasma(n=5) |
83-95% |
84-99% |
87-101% |
|
heparin plasma(n=5) |
81-92% |
83-93% |
80-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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