FineTest
SKU:EH3137
Human GIF(Gastric Intrinsic Factor) ELISA Kit
Human GIF(Gastric Intrinsic Factor) ELISA Kit
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Product Name
Human GIF(Gastric Intrinsic Factor) ELISA Kit
Alias
GIF ELISA Kit, IF ELISA Kit, IFMH ELISA Kit, INF ELISA Kit, TCN3 ELISA Kit, gastric intrinsic factor ELISA Kit, vitamin B synthesis ELISA Kit, IFIntrinsic factor ELISA Kit, IFMHgastric intrinsic factor ELISA Kit, INF ELISA Kit, TCN3 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
GIF ELISA Kit allows for the in vitro quantitative determination of GIF concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
3.125-200ng/ml
Sensitivity
< 1.875ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of GIF and the recovery rates were calculated by comparing the measured value to the expected amount of GIF in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
91-104 |
99 |
|
EDTA plasma(n=5) |
87-105 |
99 |
|
heparin plasma(n=5) |
86-103 |
94 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GIF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
88-102% |
85-103% |
88-99% |
|
EDTA plasma(n=5) |
84-96% |
85-95% |
82-99% |
|
heparin plasma(n=5) |
87-98% |
90-100% |
91-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
