FineTest
SKU:EH3122
Human GCLM(Glutamate Cysteine Ligase, Modifier Subunit) ELISA Kit
Human GCLM(Glutamate Cysteine Ligase, Modifier Subunit) ELISA Kit
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Product Name
Human GCLM(Glutamate Cysteine Ligase, Modifier Subunit) ELISA Kit
Alias
GCLM ELISA Kit, GLCLR ELISA Kit, Glutamate--cysteine ligase modifier subunit ELISA Kit, Gamma-glutamylcysteine synthetase regulatory subunit ELISA Kit, GCS light chain ELISA Kit, Gamma-ECS regulatory subunit ELISA Kit, Glutamate--cysteine ligase regulatory subunit ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
GCLM ELISA Kit allows for the in vitro quantitative determination of GCLM concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Human
UniProt ID
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of GCLM and the recovery rates were calculated by comparing the measured value to the expected amount of GCLM in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
94-103 |
98 |
|
EDTA plasma(n=5) |
92-102 |
97 |
|
heparin plasma(n=5) |
85-104 |
95 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GCLM and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
89-105% |
88-104% |
86-101% |
|
EDTA plasma(n=5) |
82-100% |
82-101% |
86-100% |
|
heparin plasma(n=5) |
80-96% |
81-100% |
80-96% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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