FineTest
SKU:EH3100
Human GAD-Ab(Anti-Glutamic Acid Decarboxylase antibody) ELISA Kit
Human GAD-Ab(Anti-Glutamic Acid Decarboxylase antibody) ELISA Kit
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Product Name
Human GAD-Ab(Anti-Glutamic Acid Decarboxylase antibody) ELISA Kit
Alias
GAD-Ab ELISA Kit
Detection Method
Sandwich ELISA, Double Antigen
Application
GAD-Ab ELISA Kit allows for the in vitro quantitative determination of GAD-Ab concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
1.563-100ng/ml
Sensitivity
< 0.938ng/ml
Species
Human
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of GAD-Ab and the recovery rates were calculated by comparing the measured value to the expected amount of GAD-Ab in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
89-99 |
95 |
|
EDTA plasma(n=5) |
85-100 |
93 |
|
heparin plasma(n=5) |
89-104 |
96 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GAD-Ab and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-105% |
87-100% |
92-102% |
|
EDTA plasma(n=5) |
86-101% |
82-97% |
87-101% |
|
heparin plasma(n=5) |
88-98% |
83-94% |
80-89% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
