FineTest
SKU:EH3092
Human F-TESTO(Free Testoterone) ELISA Kit
Human F-TESTO(Free Testoterone) ELISA Kit
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Product Name
Human F-TESTO(Free Testoterone) ELISA Kit
Alias
F-TESTO ELISA Kit, Free Testoterone ELISA Kit, 17ß-hydroxyandrost-4-ene-3-one ELISA Kit
Detection Method
Competitive ELISA, Coated with Antigen
Application
F-TESTO ELISA Kit allows for the in vitro quantitative determination of F-TESTO concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Human
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of F-TESTO and the recovery rates were calculated by comparing the measured value to the expected amount of F-TESTO in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
86-99 |
91 |
|
EDTA plasma(n=5) |
90-97 |
93 |
|
heparin plasma(n=5) |
87-99 |
93 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of F-TESTO and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
90-104% |
88-103% |
86-95% |
|
EDTA plasma(n=5) |
82-98% |
85-99% |
84-96% |
|
heparin plasma(n=5) |
82-92% |
80-99% |
82-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
