FineTest
SKU:EH2863
Human COL17(Collagen Type XVII) ELISA Kit
Human COL17(Collagen Type XVII) ELISA Kit
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Product Name
Human COL17(Collagen Type XVII) ELISA Kit
Alias
COL17 ELISA Kit, 180 kDa bullous pemphigoid antigen 2 ELISA Kit, Bullous pemphigoid antigen 2 ELISA Kit, BP180 ELISA Kit, BPAG2 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
COL17 ELISA Kit allows for the in vitro quantitative determination of COL17 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
3.125-200ng/ml
Sensitivity
< 1.875ng/ml
Species
Human
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of COL17 and the recovery rates were calculated by comparing the measured value to the expected amount of COL17 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
86-105 |
96 |
|
EDTA plasma(n=5) |
92-102 |
97 |
|
heparin plasma(n=5) |
86-102 |
93 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of COL17 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
98-105% |
89-96% |
85-103% |
|
EDTA plasma(n=5) |
86-97% |
82-95% |
91-101% |
|
heparin plasma(n=5) |
81-95% |
81-99% |
81-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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