FineTest
SKU:EH2744
Human C4d(Complement Fragment 4d) ELISA Kit
Human C4d(Complement Fragment 4d) ELISA Kit
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Product Name
Human C4d(Complement Fragment 4d) ELISA Kit
Alias
C4d ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
C4d ELISA Kit allows for the in vitro quantitative determination of C4d concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.625-40ng/ml
Sensitivity
< 0.375ng/ml
Species
Human
UniProt ID
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of C4d and the recovery rates were calculated by comparing the measured value to the expected amount of C4d in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-100 |
92 |
|
EDTA plasma(n=5) |
85-105 |
97 |
|
heparin plasma(n=5) |
96-101 |
99 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C4d and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-101% |
91-105% |
85-101% |
|
EDTA plasma(n=5) |
95-99% |
82-90% |
82-96% |
|
heparin plasma(n=5) |
80-99% |
80-95% |
82-92% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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