FineTest
SKU:EH2722
Human C/EBPα(CCAAT/Enhancer Binding Protein Alpha) ELISA Kit
Human C/EBPα(CCAAT/Enhancer Binding Protein Alpha) ELISA Kit
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Product Name
Human C/EBPα(CCAAT/Enhancer Binding Protein Alpha) ELISA Kit
Alias
C ELISA Kit, EBPα ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
C/EBPα ELISA Kit allows for the in vitro quantitative determination of C/EBPα concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Human
UniProt ID
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of C/EBPα and the recovery rates were calculated by comparing the measured value to the expected amount of C/EBPα in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
87-105 |
98 |
|
EDTA plasma(n=5) |
90-104 |
98 |
|
heparin plasma(n=5) |
85-103 |
96 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C/EBPα and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
92-102% |
87-97% |
87-95% |
|
EDTA plasma(n=5) |
82-97% |
83-98% |
84-101% |
|
heparin plasma(n=5) |
80-95% |
82-95% |
86-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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