FineTest
SKU:EH2674
Human ATF-1(Activating Transcription Factor 1) ELISA Kit
Human ATF-1(Activating Transcription Factor 1) ELISA Kit
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Product Name
Human ATF-1(Activating Transcription Factor 1) ELISA Kit
Alias
ATF-1 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
ATF-1 ELISA Kit allows for the in vitro quantitative determination of ATF-1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
1.563-100ng/ml
Sensitivity
< 0.938ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of ATF-1 and the recovery rates were calculated by comparing the measured value to the expected amount of ATF-1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
90-105 |
98 |
|
EDTA plasma(n=5) |
88-105 |
98 |
|
heparin plasma(n=5) |
91-105 |
97 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ATF-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-103% |
85-99% |
91-99% |
|
EDTA plasma(n=5) |
88-101% |
84-101% |
83-99% |
|
heparin plasma(n=5) |
80-96% |
82-93% |
85-94% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
