FineTest
SKU:EH2670
Human ASK-1(Apoptosis Signal Regulating Kinase 1) ELISA Kit
Human ASK-1(Apoptosis Signal Regulating Kinase 1) ELISA Kit
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Product Name
Human ASK-1(Apoptosis Signal Regulating Kinase 1) ELISA Kit
Alias
ASK-1 ELISA Kit, MAP3K5 ELISA Kit, MEKK5 ELISA Kit, Apoptosis signal-regulating kinase 1 ELISA Kit, ASK-1 ELISA Kit, ASK1MEKK 5 ELISA Kit, EC 2.7.11 ELISA Kit, MAP ELISA Kit, ERK kinase kinase 5 ELISA Kit, MAPK ELISA Kit, ERK kinase kinase 5 ELISA Kit, MAPKKK5EC 2.7.11.25 ELISA Kit, MEK kinase 5 ELISA Kit, MEKK5apoptosis signal regulating kinase 1 ELISA Kit, mitogen-activated protein kinase kinase kinase 5 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
ASK-1 ELISA Kit allows for the in vitro quantitative determination of ASK-1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Human
UniProt ID
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of ASK-1 and the recovery rates were calculated by comparing the measured value to the expected amount of ASK-1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-104 |
95 |
|
EDTA plasma(n=5) |
86-104 |
95 |
|
heparin plasma(n=5) |
90-102 |
96 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ASK-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
90-104% |
86-103% |
85-101% |
|
EDTA plasma(n=5) |
88-98% |
84-100% |
86-101% |
|
heparin plasma(n=5) |
84-100% |
84-100% |
80-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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