FineTest
SKU:EH2612
Human AKAP12(A Kinase Anchor Protein 12) ELISA Kit
Human AKAP12(A Kinase Anchor Protein 12) ELISA Kit
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Product Name
Human AKAP12(A Kinase Anchor Protein 12) ELISA Kit
Alias
AKAP12 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
AKAP12 ELISA Kit allows for the in vitro quantitative determination of AKAP12 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of AKAP12 and the recovery rates were calculated by comparing the measured value to the expected amount of AKAP12 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-99 |
93 |
|
EDTA plasma(n=5) |
86-101 |
93 |
|
heparin plasma(n=5) |
88-96 |
91 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AKAP12 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
87-104% |
92-104% |
85-102% |
|
EDTA plasma(n=5) |
88-101% |
90-98% |
83-101% |
|
heparin plasma(n=5) |
88-96% |
82-98% |
80-97% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
