FineTest
SKU:EH2525
Human SPAG1(Sperm-associated antigen 1) ELISA Kit
Human SPAG1(Sperm-associated antigen 1) ELISA Kit
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Product Name
Human SPAG1(Sperm-associated antigen 1) ELISA Kit
Alias
SPAG1 ELISA Kit, HSD-3.8 ELISA Kit, Infertility-related sperm protein Spag-1 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
SPAG1 ELISA Kit allows for the in vitro quantitative determination of SPAG1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of SPAG1 and the recovery rates were calculated by comparing the measured value to the expected amount of SPAG1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-97 |
92 |
|
EDTA plasma(n=5) |
88-105 |
95 |
|
heparin plasma(n=5) |
89-92 |
90 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SPAG1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
90-105% |
89-103% |
90-100% |
|
EDTA plasma(n=5) |
88-100% |
86-100% |
89-97% |
|
heparin plasma(n=5) |
86-91% |
88-99% |
95-97% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
