FineTest
SKU:EH2429
Human GPC1(Glypican-1) ELISA Kit
Human GPC1(Glypican-1) ELISA Kit
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Product Name
Human GPC1(Glypican-1) ELISA Kit
Alias
GPC1 ELISA Kit, FLJ38078 ELISA Kit, glypican ELISA Kit, glypican 1 ELISA Kit, glypican proteoglycan 1 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
GPC1 ELISA Kit allows for the in vitro quantitative determination of GPC1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
78.125-5000pg/ml
Sensitivity
< 46.875pg/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of GPC1 and the recovery rates were calculated by comparing the measured value to the expected amount of GPC1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
88-102 |
95 |
|
EDTA plasma(n=5) |
85-105 |
93 |
|
heparin plasma(n=5) |
85-103 |
94 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GPC1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
88-103% |
86-104% |
88-102% |
|
EDTA plasma(n=5) |
82-93% |
82-91% |
84-101% |
|
heparin plasma(n=5) |
84-100% |
81-94% |
84-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
