FineTest
SKU:EH2403
Human SCUBE1(Signal peptide, CUB and EGF-like domain-containing protein 1) ELISA Kit
Human SCUBE1(Signal peptide, CUB and EGF-like domain-containing protein 1) ELISA Kit
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Product Name
Human SCUBE1(Signal peptide, CUB and EGF-like domain-containing protein 1) ELISA Kit
Alias
SCUBE1 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
SCUBE1 ELISA Kit allows for the in vitro quantitative determination of SCUBE1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of SCUBE1 and the recovery rates were calculated by comparing the measured value to the expected amount of SCUBE1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-105 |
96 |
|
EDTA plasma(n=5) |
86-104 |
95 |
|
heparin plasma(n=5) |
88-97 |
92 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SCUBE1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
91-105% |
90-104% |
93-104% |
|
EDTA plasma(n=5) |
82-100% |
89-99% |
90-100% |
|
heparin plasma(n=5) |
81-100% |
87-100% |
82-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
