FineTest
SKU:EH2388
Human NEFA(Nesfatin-1) ELISA Kit
Human NEFA(Nesfatin-1) ELISA Kit
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Product Name
Human NEFA(Nesfatin-1) ELISA Kit
Alias
NEFA ELISA Kit
Detection Method
Competitive ELISA, Coated with Antigen
Application
NEFA ELISA Kit allows for the in vitro quantitative determination of NEFA concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of NEFA and the recovery rates were calculated by comparing the measured value to the expected amount of NEFA in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
89-103 |
94 |
|
EDTA plasma(n=5) |
86-99 |
95 |
|
heparin plasma(n=5) |
88-105 |
98 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NEFA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-101% |
86-100% |
87-101% |
|
EDTA plasma(n=5) |
82-98% |
90-100% |
83-99% |
|
heparin plasma(n=5) |
83-100% |
80-98% |
81-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
