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SKU:EH2386
Human TOP2A(DNA topoisomerase 2-alpha) ELISA Kit
Human TOP2A(DNA topoisomerase 2-alpha) ELISA Kit
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Product Name
Human TOP2A(DNA topoisomerase 2-alpha) ELISA Kit
Alias
TOP2A ELISA Kit, TOP2 ELISA Kit, TP2A ELISA Kit, DNA topoisomerase II ELISA Kit, alpha isozyme ELISA Kit, DNA topoisomerase ELISA Kit, ATP-hydrolyzing ELISA Kit, DNA topoisomerase 2-alpha ELISA Kit, TOP2DNA gyrase ELISA Kit, topoisomerase ELISA Kit, DNA II alpha ELISA Kit, 170kD ELISA Kit, topoisomerase ELISA Kit, DNA II alpha 170kDa ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
TOP2A ELISA Kit allows for the in vitro quantitative determination of TOP2A concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Human
UniProt ID
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of TOP2A and the recovery rates were calculated by comparing the measured value to the expected amount of TOP2A in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
87-101 |
93 |
|
EDTA plasma(n=5) |
88-105 |
97 |
|
heparin plasma(n=5) |
88-98 |
93 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TOP2A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
90-104% |
87-101% |
89-103% |
|
EDTA plasma(n=5) |
85-101% |
90-95% |
91-99% |
|
heparin plasma(n=5) |
80-99% |
84-98% |
82-96% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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