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FineTest

SKU:EH2386

Human TOP2A(DNA topoisomerase 2-alpha) ELISA Kit

Human TOP2A(DNA topoisomerase 2-alpha) ELISA Kit

Regular price $450.00 USD
Regular price Sale price $450.00 USD
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size
Sensitivity
Range

Product Name

Human TOP2A(DNA topoisomerase 2-alpha) ELISA Kit

Alias

TOP2A ELISA Kit, TOP2 ELISA Kit, TP2A ELISA Kit, DNA topoisomerase II ELISA Kit, alpha isozyme ELISA Kit, DNA topoisomerase ELISA Kit, ATP-hydrolyzing ELISA Kit, DNA topoisomerase 2-alpha ELISA Kit, TOP2DNA gyrase ELISA Kit, topoisomerase ELISA Kit, DNA II alpha ELISA Kit, 170kD ELISA Kit, topoisomerase ELISA Kit, DNA II alpha 170kDa ELISA Kit

Detection Method

Sandwich ELISA, Double Antibody

Application

TOP2A ELISA Kit allows for the in vitro quantitative determination of TOP2A concentrations in serum, plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.313-20ng/ml

Sensitivity

< 0.188ng/ml

Species

Human

UniProt ID

P11388

Standard Curve

Storage

2-8 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of TOP2A and the recovery rates were calculated by comparing the measured value to the expected amount of TOP2A in samples.

Matrix

Recovery range(%)

Average(%)

serum(n=5)

87-101

93

EDTA plasma(n=5)

88-105

97

heparin plasma(n=5)

88-98

93

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TOP2A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

serum(n=5)

90-104%

87-101%

89-103%

EDTA plasma(n=5)

85-101%

90-95%

91-99%

heparin plasma(n=5)

80-99%

84-98%

82-96%

CV(%)

Intra-Assay: CV<8%

Inter-Assay: CV<10%

Note

For Research Use Only

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