FineTest
SKU:EH2334
Human OPLAH(5-oxoprolinase) ELISA Kit
Human OPLAH(5-oxoprolinase) ELISA Kit
Couldn't load pickup availability
Product Name
Human OPLAH(5-oxoprolinase) ELISA Kit
Alias
OPLAH ELISA Kit, 5-oxo-L-prolinase ELISA Kit, 5-OPase ELISA Kit, Pyroglutamase ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
OPLAH ELISA Kit allows for the in vitro quantitative determination of OPLAH concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of OPLAH and the recovery rates were calculated by comparing the measured value to the expected amount of OPLAH in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-102 |
93 |
|
EDTA plasma(n=5) |
90-99 |
95 |
|
heparin plasma(n=5) |
88-104 |
97 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of OPLAH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
89-100% |
85-104% |
87-99% |
|
EDTA plasma(n=5) |
86-101% |
84-99% |
87-96% |
|
heparin plasma(n=5) |
81-97% |
84-99% |
80-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
