FineTest
SKU:EH2253
Human WIF1(Wnt inhibitory factor 1) ELISA Kit
Human WIF1(Wnt inhibitory factor 1) ELISA Kit
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Product Name
Human WIF1(Wnt inhibitory factor 1) ELISA Kit
Alias
WIF1 ELISA Kit, WIF-1 ELISA Kit, WNT inhibitory factor 1 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
WIF1 ELISA Kit allows for the in vitro quantitative determination of WIF1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000pg/ml
Sensitivity
< 18.75pg/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of WIF1 and the recovery rates were calculated by comparing the measured value to the expected amount of WIF1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
89-104 |
95 |
|
EDTA plasma(n=5) |
86-101 |
94 |
|
heparin plasma(n=5) |
85-105 |
98 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of WIF1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
89-99% |
89-98% |
86-105% |
|
EDTA plasma(n=5) |
91-98% |
83-98% |
83-101% |
|
heparin plasma(n=5) |
92-100% |
80-100% |
90-96% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
