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SKU:EH2217

Human HUWE1(E3 ubiquitin-protein ligase HUWE1) ELISA Kit

Human HUWE1(E3 ubiquitin-protein ligase HUWE1) ELISA Kit

Regular price $450.00 USD
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size
Sensitivity
Range

Product Name

Human HUWE1(E3 ubiquitin-protein ligase HUWE1) ELISA Kit

 

Alias

HUWE1 ELISA Kit, Upstream regulatory element-binding protein 1 ELISA Kit, URE-B1 ELISA Kit, URE-binding protein 1 ELISA Kit, Mcl-1 ubiquitin ligase E3 ELISA Kit, Large structure of UREB1 ELISA Kit, LASU1 ELISA Kit, Homologous to E6AP carboxyl terminus homologous protein 9 ELISA Kit, HectH9 ELISA Kit, ARF-binding protein 1 ELISA Kit, ARF-BP1 ELISA Kit, HECT ELISA Kit, UBA and WWE domain-containing protein 1 ELISA Kit

Detection Method

Sandwich ELISA, Double Antibody

Application

HUWE1 ELISA Kit allows for the in vitro quantitative determination of HUWE1 concentrations in serum, plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Species

Human

UniProt ID

Q7Z6Z7

Standard Curve

Storage

2-8 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of HUWE1 and the recovery rates were calculated by comparing the measured value to the expected amount of HUWE1 in samples.

Matrix

Recovery range(%)

Average(%)

serum(n=5)

87-101

95

EDTA plasma(n=5)

88-101

94

heparin plasma(n=5)

85-98

91

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HUWE1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

serum(n=5)

92-96%

87-103%

86-105%

EDTA plasma(n=5)

84-97%

82-95%

82-95%

heparin plasma(n=5)

80-99%

87-100%

82-99%

CV(%)

Intra-Assay: CV<8%

Inter-Assay: CV<10%

Note

For Research Use Only

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