FineTest
SKU:EH2195
Human SEMA3F(Semaphorin-3F) ELISA Kit
Human SEMA3F(Semaphorin-3F) ELISA Kit
Couldn't load pickup availability
Product Name
Human SEMA3F(Semaphorin-3F) ELISA Kit
Alias
SEMA3F ELISA Kit, Sema III ELISA Kit, F ELISA Kit, Semaphorin IV ELISA Kit, Sema IV ELISA Kit, Sema3F ELISA Kit, SEMA4 ELISA Kit, SEMAK ELISA Kit, sema domain ELISA Kit, immunoglobulin domain ELISA Kit, Ig ELISA Kit, short basic domain ELISA Kit, secreted ELISA Kit, semaphorin 3F ELISA Kit, sema domain ELISA Kit, immunoglobulin domain ELISA Kit, Ig ELISA Kit, short basic domain ELISA Kit, secreted ELISA Kit, 3F ELISA Kit, sema III ELISA Kit, F ELISA Kit, sema IV ELISA Kit, SEMA4 ELISA Kit, SEMA-IV ELISA Kit, semaphorin III ELISA Kit, F ELISA Kit, semaphorin IV ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
SEMA3F ELISA Kit allows for the in vitro quantitative determination of SEMA3F concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Human
UniProt ID
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of SEMA3F and the recovery rates were calculated by comparing the measured value to the expected amount of SEMA3F in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
90-101 |
96 |
|
EDTA plasma(n=5) |
88-104 |
96 |
|
heparin plasma(n=5) |
86-98 |
92 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SEMA3F and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-102% |
86-103% |
85-105% |
|
EDTA plasma(n=5) |
82-93% |
89-101% |
83-99% |
|
heparin plasma(n=5) |
82-96% |
82-100% |
81-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
Share
