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SKU:EH2196
Human SEMA4D(Semaphorin-4D) ELISA Kit
Human SEMA4D(Semaphorin-4D) ELISA Kit
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Product Name
Human SEMA4D(Semaphorin-4D) ELISA Kit
Alias
SEMA4D ELISA Kit, GR3 ELISA Kit, A8 ELISA Kit, BB18 ELISA Kit, CD100 ELISA Kit, COLL-4 ELISA Kit, M-sema-G ELISA Kit, SEMA4D ELISA Kit, SEMAJ ELISA Kit, A8 ELISA Kit, CD100 antigen ELISA Kit, CD100M-sema-G ELISA Kit, coll-4 ELISA Kit, immunoglobulin domain ELISA Kit, Ig ELISA Kit, transmembrane domain ELISA Kit, TM and shortcytoplasmic domain ELISA Kit, 4D ELISA Kit, sema domain ELISA Kit, immunoglobulin domain ELISA Kit, Ig ELISA Kit, transmembrane domain ELISA Kit, TM and shortcytoplasmic domain ELISA Kit, semaphorin 4D ELISA Kit, semaphorin-4D ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
SEMA4D ELISA Kit allows for the in vitro quantitative determination of SEMA4D concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
125-8000pg/ml
Sensitivity
< 75pg/ml
Species
Human
UniProt ID
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of SEMA4D and the recovery rates were calculated by comparing the measured value to the expected amount of SEMA4D in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
87-103 |
95 |
|
EDTA plasma(n=5) |
92-104 |
100 |
|
heparin plasma(n=5) |
89-105 |
96 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SEMA4D and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-100% |
86-100% |
86-101% |
|
EDTA plasma(n=5) |
84-95% |
82-96% |
84-101% |
|
heparin plasma(n=5) |
84-100% |
82-100% |
80-98% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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