FineTest
SKU:EH2189
Human NEFH(Neurofilament heavy polypeptide) ELISA Kit
Human NEFH(Neurofilament heavy polypeptide) ELISA Kit
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Product Name
Human NEFH(Neurofilament heavy polypeptide) ELISA Kit
Alias
Human NEFH ELISA Kit, NEFH neurofilament ELISA Kit, heavy polypeptide ELISA Kit, neurofilament ELISA Kit, heavy polypeptid ELISA Kit, NFH ELISA Kit, 200 kDa neurofilament protein ELISA Kit, Neurofilament triplet H protein ELISA Kit, NF-H ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
NEFH ELISA Kit allows for the in vitro quantitative determination of NEFH concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Human
UniProt ID
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of NEFH and the recovery rates were calculated by comparing the measured value to the expected amount of NEFH in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
89-96 |
92 |
|
EDTA plasma(n=5) |
85-98 |
91 |
|
heparin plasma(n=5) |
85-104 |
96 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NEFH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
88-101% |
89-103% |
87-104% |
|
EDTA plasma(n=5) |
86-98% |
83-98% |
82-96% |
|
heparin plasma(n=5) |
80-97% |
81-91% |
82-94% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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