FineTest
SKU:EH2186
Human SEPT5(Septin-5) ELISA Kit
Human SEPT5(Septin-5) ELISA Kit
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Product Name
Human SEPT5(Septin-5) ELISA Kit
Alias
SEPT5 ELISA Kit, Cell division control-related protein 1 ELISA Kit, CDCrel-1 ELISA Kit, Peanut-like protein 1 ELISA Kit, PNUTL1 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
SEPT5 ELISA Kit allows for the in vitro quantitative determination of Septin-5 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.781-50ng/ml
Sensitivity
< 0.469ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Septin-5 and the recovery rates were calculated by comparing the measured value to the expected amount of Septin-5 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
94-103 |
98 |
|
EDTA plasma(n=5) |
86-100 |
92 |
|
heparin plasma(n=5) |
86-103 |
94 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Septin-5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-103% |
92-100% |
96-105% |
|
EDTA plasma(n=5) |
90-101% |
83-98% |
83-95% |
|
heparin plasma(n=5) |
80-100% |
86-94% |
84-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
