FineTest
SKU:EH2174
Human APLN(Apelin) ELISA Kit
Human APLN(Apelin) ELISA Kit
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Product Name
Human APLN(Apelin) ELISA Kit
Alias
APLN ELISA Kit, Apelin ELISA Kit, APEL ELISA Kit, XNPEP2 ELISA Kit, APJ endogenous ligand ELISA Kit
Detection Method
Competitive ELISA, Coated with Antigen
Application
APLN ELISA Kit allows for the in vitro quantitative determination of APLN concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
62.5-4000pg/ml
Sensitivity
< 37.5pg/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of APLN and the recovery rates were calculated by comparing the measured value to the expected amount of APLN in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
86-97 |
91 |
|
EDTA plasma(n=5) |
86-96 |
93 |
|
heparin plasma(n=5) |
91-99 |
95 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of APLN and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-103% |
85-104% |
85-99% |
|
EDTA plasma(n=5) |
93-99% |
87-101% |
84-101% |
|
heparin plasma(n=5) |
81-100% |
83-96% |
84-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
