FineTest
SKU:EH2171
Human TPH2(Tryptophan 5-hydroxylase 2) ELISA Kit
Human TPH2(Tryptophan 5-hydroxylase 2) ELISA Kit
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Product Name
Human TPH2(Tryptophan 5-hydroxylase 2) ELISA Kit
Alias
TPH2 ELISA Kit, Neuronal tryptophan hydroxylase ELISA Kit, Tryptophan 5-monooxygenase 2 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
TPH2 ELISA Kit allows for the in vitro quantitative determination of TPH2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of TPH2 and the recovery rates were calculated by comparing the measured value to the expected amount of TPH2 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
89-104 |
98 |
|
EDTA plasma(n=5) |
89-105 |
98 |
|
heparin plasma(n=5) |
86-98 |
91 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TPH2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
90-102% |
89-101% |
85-100% |
|
EDTA plasma(n=5) |
89-98% |
84-96% |
84-99% |
|
heparin plasma(n=5) |
80-98% |
83-98% |
88-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
