FineTest
SKU:EH2148
Human Smad3/MADH3(Mothers Against Decapentaplegic Homolog 3) ELISA Kit
Human Smad3/MADH3(Mothers Against Decapentaplegic Homolog 3) ELISA Kit
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Product Name
Human Smad3/MADH3(Mothers Against Decapentaplegic Homolog 3) ELISA Kit
Alias
Smad3 ELISA Kit, MADH3 ELISA Kit, JV15-2 ELISA Kit, SMAD family member 3 ELISA Kit, hMAD-3 ELISA Kit, hSMAD3 ELISA Kit, MAD homolog 3 ELISA Kit, mad protein homolog ELISA Kit, MAD ELISA Kit, mothers against decapentaplegic homolog 3 ELISA Kit, MAD ELISA Kit, mothers against decapentaplegic homolog 3 ELISA Kit, Drosophila ELISA Kit, mad3 ELISA Kit, Mad3 ELISA Kit, MADH3mad homolog JV15-2 ELISA Kit, mothers against decapentaplegic homolog 3 ELISA Kit, Mothers against DPP homolog 3 ELISA Kit, SMA- and MAD-related protein 3 ELISA Kit, SMAD family member 3HSPC193 ELISA Kit, SMAD ELISA Kit, mothers against DPP homolog 3 ELISA Kit, SMAD ELISA Kit, mothers against DPP homolog 3 ELISA Kit, Drosophila ELISA Kit, Smad3 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
Smad3/MADH3 ELISA Kit allows for the in vitro quantitative determination of Smad3/MADH3 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.625-40ng/ml
Sensitivity
< 0.375ng/ml
Species
Human
UniProt ID
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of Smad3/MADH3 and the recovery rates were calculated by comparing the measured value to the expected amount of Smad3/MADH3 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
86-101 |
95 |
|
EDTA plasma(n=5) |
89-105 |
96 |
|
heparin plasma(n=5) |
86-102 |
92 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Smad3/MADH3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
91-101% |
92-103% |
88-100% |
|
EDTA plasma(n=5) |
94-101% |
85-97% |
85-99% |
|
heparin plasma(n=5) |
80-83% |
89-100% |
82-92% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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