FineTest
SKU:EH2146
Human WNT3A(Protein Wnt-3a) ELISA Kit
Human WNT3A(Protein Wnt-3a) ELISA Kit
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Product Name
Human WNT3A(Protein Wnt-3a) ELISA Kit
Alias
WNT3A ELISA Kit, MGC119418 ELISA Kit, MGC119419 ELISA Kit, MGC119420 ELISA Kit, protein Wnt-3a ELISA Kit, wingless-type MMTV integRation site family ELISA Kit, member 3A ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
WNT3A ELISA Kit allows for the in vitro quantitative determination of WNT3A concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000pg/ml
Sensitivity
< 18.75pg/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of WNT3A and the recovery rates were calculated by comparing the measured value to the expected amount of WNT3A in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
88-103 |
98 |
|
EDTA plasma(n=5) |
88-95 |
91 |
|
heparin plasma(n=5) |
91-105 |
97 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of WNT3A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
85-105% |
85-98% |
85-97% |
|
EDTA plasma(n=5) |
84-101% |
83-98% |
82-101% |
|
heparin plasma(n=5) |
86-100% |
83-100% |
80-96% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
