FineTest
SKU:EH2110
Human 5-HETE(5-Hydroxyeicosatetraenoic Acid) ELISA Kit
Human 5-HETE(5-Hydroxyeicosatetraenoic Acid) ELISA Kit
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Product Name
Human 5-HETE(5-Hydroxyeicosatetraenoic Acid) ELISA Kit
Alias
5-HETE ELISA Kit, 5-Hydroxyeicosatetraenoic Acid ELISA Kit
Detection Method
Competitive ELISA, Coated with Antigen
Application
5-HETE ELISA Kit allows for the in vitro quantitative determination of 5-HETE concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Human
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of 5-HETE and the recovery rates were calculated by comparing the measured value to the expected amount of 5-HETE in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-104 |
93 |
|
EDTA plasma(n=5) |
87-104 |
99 |
|
heparin plasma(n=5) |
90-103 |
94 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 5-HETE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
88-102% |
87-98% |
85-102% |
|
EDTA plasma(n=5) |
83-97% |
94-100% |
84-97% |
|
heparin plasma(n=5) |
85-95% |
82-92% |
85-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
