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SKU:EH2080

Human 4-HNE(4-Hydroxynonenal) ELISA Kit

Human 4-HNE(4-Hydroxynonenal) ELISA Kit

Regular price $450.00 USD
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size
Sensitivity
Range

Product Name

Human 4-HNE(4-Hydroxynonenal) ELISA Kit

 

Alias

4-HNE ELISA Kit, 4-Hydroxynonenal ELISA Kit, HNE ELISA Kit, 4 HNE ELISA Kit

Detection Method

Competitive ELISA, Coated with Antigen

Application

4-HNE ELISA Kit allows for the in vitro quantitative determination of 4-HNE concentrations in serum, plasma, tissue homogenates and other biological fluids.

Size

96T

Range

31.25-2000pg/ml

Sensitivity

< 18.75pg/ml

Species

Human

Standard Curve

Storage

2-8 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of 4-HNE and the recovery rates were calculated by comparing the measured value to the expected amount of 4-HNE in samples.

Matrix

Recovery range(%)

Average(%)

serum(n=5)

86-103

93

EDTA plasma(n=5)

90-104

97

heparin plasma(n=5)

85-101

93

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 4-HNE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

serum(n=5)

89-105%

87-102%

86-104%

EDTA plasma(n=5)

85-100%

83-95%

87-101%

heparin plasma(n=5)

81-98%

84-100%

91-99%

CV(%)

Intra-Assay: CV<8%

Inter-Assay: CV<10%

Note

For Research Use Only

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