FineTest
SKU:EH2080
Human 4-HNE(4-Hydroxynonenal) ELISA Kit
Human 4-HNE(4-Hydroxynonenal) ELISA Kit
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Product Name
Human 4-HNE(4-Hydroxynonenal) ELISA Kit
Alias
4-HNE ELISA Kit, 4-Hydroxynonenal ELISA Kit, HNE ELISA Kit, 4 HNE ELISA Kit
Detection Method
Competitive ELISA, Coated with Antigen
Application
4-HNE ELISA Kit allows for the in vitro quantitative determination of 4-HNE concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000pg/ml
Sensitivity
< 18.75pg/ml
Species
Human
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of 4-HNE and the recovery rates were calculated by comparing the measured value to the expected amount of 4-HNE in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
86-103 |
93 |
|
EDTA plasma(n=5) |
90-104 |
97 |
|
heparin plasma(n=5) |
85-101 |
93 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 4-HNE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
89-105% |
87-102% |
86-104% |
|
EDTA plasma(n=5) |
85-100% |
83-95% |
87-101% |
|
heparin plasma(n=5) |
81-98% |
84-100% |
91-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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