FineTest
SKU:EH2068
Human DEFB127(Beta-defensin 127) ELISA Kit
Human DEFB127(Beta-defensin 127) ELISA Kit
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Product Name
Human DEFB127(Beta-defensin 127) ELISA Kit
Alias
DEFB127 ELISA Kit, Beta-defensin 127 ELISA Kit, Beta-defensin 27 ELISA Kit, DEFB-27 ELISA Kit, Defensin ELISA Kit, beta 127 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
DEFB127 ELISA Kit allows for the in vitro quantitative determination of DEFB127 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
93.75-6000pg/ml
Sensitivity
< 56.25pg/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of DEFB127 and the recovery rates were calculated by comparing the measured value to the expected amount of DEFB127 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-96 |
92 |
|
EDTA plasma(n=5) |
85-97 |
91 |
|
heparin plasma(n=5) |
86-104 |
96 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of DEFB127 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
87-103% |
85-102% |
87-103% |
|
EDTA plasma(n=5) |
82-101% |
83-101% |
86-99% |
|
heparin plasma(n=5) |
82-100% |
86-100% |
80-96% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
