FineTest
SKU:EH2063
Human OB(Obestatin) ELISA Kit
Human OB(Obestatin) ELISA Kit
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Product Name
Human OB(Obestatin) ELISA Kit
Alias
OB ELISA Kit, Obestatin ELISA Kit
Detection Method
Competitive ELISA, Coated with Antigen
Application
OB ELISA Kit allows for the in vitro quantitative determination of OB concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of OB and the recovery rates were calculated by comparing the measured value to the expected amount of OB in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-104 |
95 |
|
EDTA plasma(n=5) |
86-101 |
93 |
|
heparin plasma(n=5) |
89-105 |
98 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of OB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
89-99% |
85-104% |
86-103% |
|
EDTA plasma(n=5) |
83-100% |
88-93% |
82-98% |
|
heparin plasma(n=5) |
81-100% |
81-96% |
82-100% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
