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SKU:EH2056

Human MGMT(Methylated-DNA--protein-cysteine methyltransferase) ELISA Kit

Human MGMT(Methylated-DNA--protein-cysteine methyltransferase) ELISA Kit

Regular price $450.00 USD
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size
Sensitivity
Range

Product Name

Human MGMT(Methylated-DNA-protein-cysteine methyltransferase) ELISA Kit

 

Alias

MGMT ELISA Kit, Methylated-DNA--protein-cysteine methyltransferase ELISA Kit, 6-O-methylguanine-DNA methyltransferase ELISA Kit, MGMT ELISA Kit, O-6-methylguanine-DNA-alkyltransferase ELISA Kit

Detection Method

Sandwich ELISA, Double Antibody

Application

MGMT ELISA Kit allows for the in vitro quantitative determination of MGMT concentrations in serum, plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Species

Human

UniProt ID

P16455

Standard Curve

Storage

2-8 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of MGMT and the recovery rates were calculated by comparing the measured value to the expected amount of MGMT in samples.

Matrix

Recovery range(%)

Average(%)

serum(n=5)

92-102

97

EDTA plasma(n=5)

89-104

96

heparin plasma(n=5)

92-102

99

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MGMT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

serum(n=5)

86-99%

88-100%

85-104%

EDTA plasma(n=5)

85-101%

82-99%

82-89%

heparin plasma(n=5)

80-96%

84-98%

81-92%

CV(%)

Intra-Assay: CV<8%

Inter-Assay: CV<10%

Note

For Research Use Only

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