FineTest
SKU:EH2056
Human MGMT(Methylated-DNA--protein-cysteine methyltransferase) ELISA Kit
Human MGMT(Methylated-DNA--protein-cysteine methyltransferase) ELISA Kit
Couldn't load pickup availability
Product Name
Human MGMT(Methylated-DNA-protein-cysteine methyltransferase) ELISA Kit
Alias
MGMT ELISA Kit, Methylated-DNA--protein-cysteine methyltransferase ELISA Kit, 6-O-methylguanine-DNA methyltransferase ELISA Kit, MGMT ELISA Kit, O-6-methylguanine-DNA-alkyltransferase ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
MGMT ELISA Kit allows for the in vitro quantitative determination of MGMT concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Human
UniProt ID
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of MGMT and the recovery rates were calculated by comparing the measured value to the expected amount of MGMT in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
92-102 |
97 |
|
EDTA plasma(n=5) |
89-104 |
96 |
|
heparin plasma(n=5) |
92-102 |
99 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MGMT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-99% |
88-100% |
85-104% |
|
EDTA plasma(n=5) |
85-101% |
82-99% |
82-89% |
|
heparin plasma(n=5) |
80-96% |
84-98% |
81-92% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
Share
