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SKU:EH2006

Human GAPDH(Glyceraldehyde-3-phosphate dehydrogenase) ELISA Kit

Human GAPDH(Glyceraldehyde-3-phosphate dehydrogenase) ELISA Kit

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size
Sensitivity
Range

Product Name

Human GAPDH(Glyceraldehyde-3-phosphate dehydrogenase) ELISA Kit

 

Alias

GAPDH ELISA Kit, Glyceraldehyde-3-phosphate dehydrogenase ELISA Kit, Peptidyl-cysteine S-nitrosylase GAPDH ELISA Kit, GAPD ELISA Kit, G3PD ELISA Kit, G3PDH ELISA Kit, aging-associated gene 9 protein ELISA Kit

Detection Method

Sandwich ELISA, Double Antibody

Application

GAPDH ELISA Kit allows for the in vitro quantitative determination of GAPDH concentrations in serum, plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.156-10ng/ml

Sensitivity

< 0.094ng/ml

Species

Human

UniProt ID

P04406

Standard Curve

Storage

2-8 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of GAPDH and the recovery rates were calculated by comparing the measured value to the expected amount of GAPDH in samples.

Matrix

Recovery range(%)

Average(%)

serum(n=5)

94-99

97

EDTA plasma(n=5)

89-105

98

heparin plasma(n=5)

89-104

98

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GAPDH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

serum(n=5)

88-101%

87-97%

92-102%

EDTA plasma(n=5)

82-97%

86-101%

85-98%

heparin plasma(n=5)

85-98%

80-98%

84-99%

CV(%)

Intra-Assay: CV<8%

Inter-Assay: CV<10%

Note

For Research Use Only

 

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