FineTest
SKU:EH1988
Human IDE(Insulin-degrading enzyme) ELISA Kit
Human IDE(Insulin-degrading enzyme) ELISA Kit
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Product Name
Human IDE(Insulin-degrading enzyme) ELISA Kit
Alias
IDE ELISA Kit, Insulin-degrading enzyme ELISA Kit, Abeta-degrading protease ELISA Kit, Insulin protease ELISA Kit, Insulinase ELISA Kit, Insulysin ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
IDE ELISA Kit allows for the in vitro quantitative determination of IDE concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
62.5-4000pg/ml
Sensitivity
< 37.5pg/ml
Species
Human
UniProt ID
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of IDE and the recovery rates were calculated by comparing the measured value to the expected amount of IDE in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
92-100 |
95 |
|
EDTA plasma(n=5) |
90-105 |
97 |
|
heparin plasma(n=5) |
87-104 |
96 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IDE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
90-105% |
85-100% |
92-101% |
|
EDTA plasma(n=5) |
83-98% |
86-98% |
82-97% |
|
heparin plasma(n=5) |
81-96% |
83-94% |
84-92% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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