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EH1980

Human 12-LO(Arachidonate 12-Lipoxygenase) ELISA Kit

Human 12-LO(Arachidonate 12-Lipoxygenase) ELISA Kit

$450.00 USD
$450.00 USD
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size
Sensitivity
Range

Product Name

Human 12-LO(Arachidonate 12-Lipoxygenase) ELISA Kit

 

Alias

12-LO ELISA Kit, Arachidonate 12-Lipoxygenase ELISA Kit, ALOX12 ELISA Kit, LOG12 ELISA Kit, 12S-LOX ELISA Kit, 12S-lipoxygenase ELISA Kit, Platelet-type lipoxygenase 12 ELISA Kit, Platelet-type lipoxygenase 12 ELISA Kit

Detection Method

Sandwich ELISA, Double Antibody

Application

12-LO ELISA Kit allows for the in vitro quantitative determination of 12-LO concentrations in serum, plasma, tissue homogenates and other biological fluids.

Size

96T

Range

0.313-20ng/ml

Sensitivity

< 0.188ng/ml

Species

Human

UniProt ID

P18054

Standard Curve

Storage

2-8 ℃ for 6 months

Recovery

Matrices listed below were spiked with certain level of 12-LO and the recovery rates were calculated by comparing the measured value to the expected amount of 12-LO in samples.

Matrix

Recovery range(%)

Average(%)

serum(n=5)

85-105

97

EDTA plasma(n=5)

86-101

94

heparin plasma(n=5)

87-99

95

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 12-LO and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample

1:2

1:4

1:8

serum(n=5)

86-97%

85-105%

85-102%

EDTA plasma(n=5)

82-94%

88-96%

83-101%

heparin plasma(n=5)

80-99%

81-96%

80-100%

CV(%)

Intra-Assay: CV<8%

Inter-Assay: CV<10%

Note

For Research Use Only

 

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