FineTest
SKU:EH1975
Human ERCC1(DNA excision repair protein ERCC-1) ELISA Kit
Human ERCC1(DNA excision repair protein ERCC-1) ELISA Kit
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Product Name
Human ERCC1(DNA excision repair protein ERCC-1) ELISA Kit
Alias
ERCC1 ELISA Kit, Excision Repair Cross Complementing Rodent Repair Deficiency Complementation 1 ELISA Kit, DNA excision repair protein ERCC-1 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
ERCC1 ELISA Kit allows for the in vitro quantitative determination of ERCC1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of ERCC1 and the recovery rates were calculated by comparing the measured value to the expected amount of ERCC1 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
85-103 |
94 |
|
EDTA plasma(n=5) |
87-103 |
94 |
|
heparin plasma(n=5) |
85-105 |
96 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ERCC1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
86-100% |
86-105% |
87-103% |
|
EDTA plasma(n=5) |
82-101% |
86-94% |
83-98% |
|
heparin plasma(n=5) |
87-95% |
81-98% |
85-99% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
