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SKU:EH1968
Human HLA-B27(HLA class I histocompatibility antigen, B-27 alpha chain) ELISA Kit
Human HLA-B27(HLA class I histocompatibility antigen, B-27 alpha chain) ELISA Kit
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Product Name
Human HLA-B27(HLA class I histocompatibility antigen, B-27 alpha chain) ELISA Kit
Alias
HLA-B27 ELISA Kit, HLA class I histocompatibility antigen ELISA Kit, B-27 alpha chain ELISA Kit, HLAB ELISA Kit, MHC class I antigen B27 ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
HLA-B27 ELISA Kit allows for the in vitro quantitative determination of HLA-B27 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.313-20ng/ml
Sensitivity
< 0.188ng/ml
Species
Human
UniProt ID
Standard Curve

Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of HLA-B27 and the recovery rates were calculated by comparing the measured value to the expected amount of HLA-B27 in samples.
|
Matrix |
Recovery range(%) |
Average(%) |
|
serum(n=5) |
88-104 |
96 |
|
EDTA plasma(n=5) |
88-103 |
96 |
|
heparin plasma(n=5) |
88-104 |
96 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HLA-B27 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
|
Sample |
1:2 |
1:4 |
1:8 |
|
serum(n=5) |
88-102% |
85-104% |
88-103% |
|
EDTA plasma(n=5) |
89-98% |
85-100% |
82-98% |
|
heparin plasma(n=5) |
88-97% |
84-99% |
80-92% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only
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